Antifungal Susceptibility Testing of Lipopeptides Against Filamentous Fungi

The antifungal susceptibility testing of lipopeptides against filamentous fungi was performed according to the broth microdilution methodology, as described previously (Schwalbe et al., 2007). In this set of experiments, Candida krusei ATCC 6258 and amphotericin B were used to perform quality control tests for validation. As mentioned above, the test medium used was RPMI-1640 broth with MOPS buffer. Tests were performed in U-shaped 96-well plates in triplicates and the OD_450nm was recorded after 7 days of incubation at 30°C. The fungi tested were Rhizopus stolonifer IMI 017314 and Paecilomyces variotii and Byssochamys fulva IMI 040021.