15 µL of perfluorodecyltriethoxysilane (Sigma-Aldrich) was added to the bottom of a desiccator. O2 plasma-treated glass slides (2 × 2 cm2) were affixed to the lid of the desiccator using a Kapton tape. The desiccator was then evacuated using a vacuum line until a pressure of 0.05 mbar was reached, and then, the desiccator was closed. The evaporation of the silane upon evacuation was confirmed from the appearance of bubbles at the bottom of the desiccator. The hydrophobic silane was then allowed to self-assemble on the glass slides for 24 h. After assembly, the hydrophobicity of the glass slides was analyzed using contact angle measurements.
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