3.3. Preparation of MGC-g-CD-ic-TCS Hydrogel

YM Young Jae Moon
SY Sun-Jung Yoon
JK Jeung-Hyun Koo
YY Yihyun Yoon
HB Hye Jun Byun
HK Hyeon Soo Kim
GK Gilson Khang
HC Heung Jae Chun
DY Dae Hyeok Yang
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The hydrogel was prepared on a clean bench. Aqueous MGC-g-CD-ic-TCS solution was filtered using a 0.22 µm syringe filter for the removal of microorganisms, and then lyophilized. To a solution of MGC-g-CD-ic-TCS (200 mg) in DPBS (3 mL), aqueous riboflavin (12 µM; DPBS) was added, and the sample was vortexed gently. MGC-g-CD-ic-TCS was stabilized in a refrigerator (4 °C) for stabilization before use. For hydrogelation, the hydrogel precursor solution was irradiated for 200 s using a blue light (430–485 nm, 2100 mW/cm2, light-emitting diode curing light, Foshan Keyuan Medical Equipment Co., Ltd., Foshan, China). The behavior was calculated by the ratio of swollen weight to initial weight at each time interval.

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