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The ALP activity was detected after 21 and 28 days of cell culture to evaluate the initial differentiation of cAD-MSCs into preosteoblasts. The colorimetric Abcam’s Alkaline phosphates kit (Abcam, Cambridge, UK) was utilized to identify the intracellular and extracellular ALP activities.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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