Particle size, polydispersity index, and zeta potential were measured using the Dynamic Light Scattering (DLS) technique which scattered at the angle of 173° and temperature of 25 °C. This process was carried out using the Malvern Nano ZS90 (Malvern Instrument, Malvern, UK). Niosome formulation was diluted with PBS (1:100) and injected into the sample cell. The measurement was repeated in triplicate. The calculation of zeta potential was performed based on the measurement of the electrophoretic mobility of dispersed particles in a charged field.
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