4.10. Measurement of Optical Density

The Pannoramic section scanner was used to image the tissue section. CaseViewer2.2 scanning software (The digital pathology company, Hungary) was used to select the target area of the tissue for imaging. During imaging, the whole field of vision was filled as much as possible to ensure consistent background light for each photo. After imaging, Image-Pro Plus 6.0 analysis software (CAD/CAM Services Inc., Celina, TX, USA) was used to measure the positive integrated optical density (IOD) values of the three fields in each tissue slice expressed as pixel area. The corresponding positive pixel area (area) was calculated as follows: AOD value = IOD/Area. GraphPad Prism6 software (GraphPad, San Diego, CA, USA) was used to generate a line graph analysis of the AOD value.