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2.11. IL-6 ELISA Assay

VG Viviana di Giacomo
LR Lucia Recinella
AC Annalisa Chiavaroli
GO Giustino Orlando
AC Amelia Cataldi
MR Monica Rapino
VV Valentina Di Valerio
MP Matteo Politi
MA Marco Daniel Antolini
AA Alessandra Acquaviva
FB Francesco Bacchin
MM Massimo Di Mascio
SL Sheila Leone
LB Luigi Brunetti
LM Luigi Menghini
SC Simone Carradori
GZ Gokhan Zengin
GA Gunes Ak
CF Claudio Ferrante
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At each time point, the supernatants of HaCat cells were harvested, and the secretion of interleukin-6 (IL-6) in the culture media was evaluated by an ELISA kit (both from Enzo Life Sciences, Farmingdale, NY, USA), according to the manufacturer’s instructions. The optical density values were obtained by measuring the absorbance at 450 using a Multiscan GO microplate spectrophotometer (Thermo Fisher Scientific).

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