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Paraformaldehyde-fixed paraffin-embedded tissues were sectioned into 20 μm sections and stored at room temperature. Slides were the blocked for nonspecific binding using protein block, and endogenous peroxidase activity was quenched [23]. For DAB-enhanced Perls staining, slides were immersed for 1-h in 1% potassium ferricyanide in 0.1-M HCl buffer and then stained with DAB. All slides were counterstained with hematoxylin and then visualized using a Nikon Eclipse TE2000-Umicroscope (Nikon, UK).
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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