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Cell culture

FA Fadi Almouhanna
BB Biljana Blagojevic
SC Suzan Can
AG Ali Ghanem
SW Stefan Wölfl
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All cell lines used in this study (MDA MB 231, MDA MB 468, HCC 1443, T47-D, MCF7, LnCap, PC3, and DU145) were kept in a humidified cell culture incubator at 37 °C with 5% CO2. Cells were also culture in Dulbecco’s Modified Eagle Medium (DMEM)-GlutaMAX™ (Gibco, Germany), supplemented with 10% FCS (v/v) (Gibco, Germany), 1% penicillin/streptomycin (v/v) (Gibco, Germany). MDA MB 468, HCC 1443, and T47-D are kind gifts from Dr. Stefan Wiemann (DKFZ, Heidelberg). Indicated drugs were added to the standard culture medium for the indicated time points. PKM2 allosteric activators DASA-58 and TEPP-46 (Cayman Chemical-Biomol GmbH, Germany), dehydroepiandrosterone (DHEA), CBR-5884, AZD-3965, metformin, and sodium oxamate (Cayman Chemical-Biomol GmbH, Germany) 2-deoxyglucose (Fluka-Sigma-Aldrich, Germany) Rotenon, carbonyl cyanide 3-chlorophenylhydrazone (CCCP), tamoxifen, oligomycin, and sodium dichloroacetate (Sigma Aldrich, Germany).

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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