2.8. Wound-healing and matrigel invasion assays

For a microscope wound-healing migration assay, ovarian cancer cells were plated into 6-well plates and cultured to about 95% confluency. Then, a scratch was placed in the monolayer cells with a sterile pipette tip. The cells were observed at 0 and 48 h after the scraping. The average percentage of the gap closure in each group was compared.

The invasion of ovarian cancer cells was evaluated by matrigel invasion assay. Briefly, the transwell chamber was coated with matrigel basement membrane matrix on the upper surface (BD Biosciences). Then, a total of 1 × 10⁵ ovarian cancer cells were seeded into the top chamber wells and cultured in a serum-free medium for 48 h. The penetrated cells on the lower side of the filter were then fixed with paraformaldehyde and stained with crystal violet. At last, the results were evaluated with a microscope.