Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Larval Development

LS Luciana Barboza Silva
RS Reneton Gomes de Souza
SS Sandra Ribeiro da Silva
AF Alisson da Costa Feitosa
EL Elainy Cristina Lopes
SL Stelio Bezerra Pinheiro Lima
LD Leilane Rocha Barros Dourado
BP Bruno Ettore Pavan
request Request a Protocol
ask Ask a question
Favorite

Fifty newly hatched larvae were transferred to plastic containers (30 × 15 × 10 cm) covered with ‘voil’ fabric for aeration. Each container received 30 g of diet and 10 g of Sechium edule (Jacq.) Swartz, 1800, as a source of moisture, replaced every 2 d. Quantitative evaluations of the larvae occurred every 14 d, for a period of 93 d. Adults were transferred to a new container with new diet to evaluate oviposition for 30 d. The evaluated parameters were: larval weight, development period (days), survival (%), pupal number (N), total of emerged adults (N), efficiency of larva/adult formation, total of F1 generation larvae, and the instantaneous population growth rate.

Copyright and License information:  ©2021 The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A