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Cholesterol efflux was measured with a cholesterol efflux assay kit (ab196985, Abcam). BMDMs were incubated with Labeling Reagent + Equilibration Buffer mix containing ox-LDL (50 μg/mL) in the absence or presence of the LXR agonist GW3965 HCL (Selleck) at 2 μM as required. After overnight incubation, the cells were treated with either HDL (50 μg/mL) or ApoAI (10 μg/mL). Fluorescence intensity was quantified in the medium and in cells.
Copyright and License information: ©2021 Lv, Wang, Cui, Liu, Zhang, Lu, Li, Yong, Liu, Zhang, Zhang, Zhang, Li, Nan, Zhang, Guo, Wang, Chen and Bian.
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