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Systemic cytokine responses in mice infected with T. pallidum, including serum levels of interleukin (IL)-2, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ, were evaluated on days 1, 11, and 151 and analyzed using a cytometric bead array (mouse Th1 Panel [5-Plex] kit; BD Biosciences, USA) according to the manufacturer’s protocol. Data were acquired on a BD FACS Canto II flow cytometer (Becton Dickinson, USA) and analyzed using FCAP Array software (Becton Dickinson, USA).
Copyright and License information: ©2021 Lu, Zheng, Wang, Xu, Xie, Yuan, Wang and Wu
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