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RNA from rat Schwann cell cultures or mouse nerve tissue was extracted using an RNeasy Micro Extraction Kit (Qiagen). RNA quality and concentration was determined after extraction using a nanodrop 2000 machine (Thermo). One μg of RNA was converted to cDNA using SuperScriptTM II Reverse Transcriptase (Invitrogen) as per the manufacturer’s instructions. Samples were run with PrecisionPLUS qPCR Mastermix with SYBR Green (Primerdesign) with primers as described in Benito et al., 2017. Ct values were normalized to housekeeping gene expression (GAPDH and calnexin).
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