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Lactate dehydrogenase (LDH) release assay

MK Myung Hee Kim
DK Do-Hun Kim
SY Su Geun Yang
DK Dae Yu Kim
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Cell cytotoxicities were determined by the LDH released from damaged cells using the cytotoxicity LDH assay kit. In brief, ARPE-19 cells were placed in 96-well plates (5 × 103 cells/well) and pretreated with different concentrations of TPP-Niacin for 2 h, followed by adding to 300 μM H2O2 in TPP-Niacin contained media for 24 h. The background of LDH in the growth medium was measured and subtracted from all test samples. The basal levels of LDH (0% cell death), as measured in the supernatants of the vehicle-treated cells, and maximal levels of LDH (100% cell death) measured by lysis buffer induction for complete cell death, were averaged and used to calculate the percentage of cell death as per the manufacturer’s protocol.

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