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Aldefluor Assay and ALDH+/− Cell Sorting

XG Xiong Guo
LL Ling Liu
QZ Qi Zhang
WY Weiming Yang
YZ Yang Zhang
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The Aldefluor kit (Stem Cell Technologies, Vancouver, BC, Canada) was used to determine the proportion of high ALDH enzyme viable cells. In the procedure, 1 × 106 cells in each group were resuspended n Aldefluor assay buffer containing ALDH substrate. After incubation at 37°C, the cells were centrifuged and resuspended in 500 µl Aldefluor buffer and then analyzed with Navios flow cytometer, where green fluorescent FL1 analysis indicated the number of ALDH+ cells.

Copyright and License information:  ©2021 Guo, Liu, Zhang, Yang and Zhang
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