Thioflavin S staining of protein aggregates in the brain was performed using a previously described method [23]. Brain sections were incubated with 70% ethanol (Fisher, #A40520) for 1 min and then 80% ethanol for 1 min. Slides were then incubated with 0.1% Thioflavin S (Sigma, #T1892) in 80% ethanol for 15 min. Slides were then rinsed with 80% ethanol for 1 min, 70% ethanol for 1 min, and twice with distilled water. Slides were mounted with Cytoseal 60 Medium (Richard-Allan Scientific, #8310-16), and images were acquired using a fluorescence microscope equipped with the ZEN 2.5 software (Carl Zeiss, Jena, Germany).
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