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Cell culture plates were coated with poly-HEMA (P3932; Sigma-Aldrich, St. Louis, USA), a non-adhesive substratum, and allowed to evaporate to dryness at room temperature. Cells were kept in suspension by using poly-HEMA coated plates to prevent adhesion. After 48 h of suspension, cells were harvested for cell viability analysis by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and cell apoptosis analysis by flow cytometry.
Copyright and License information: ©2021 Liu, Fu, Bian, Fu, Xin, Liang, Li, Zhao, Fang, Li, Zhang, Dionigi and Sun
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