Quantification of Ki67+, BrdU+, and DCX+ Cells Along the Longitudinal Axis (by Bregma)

For anterior/posterior analysis of Ki67+, BrdU+, and DCX+ cell number, results were divided into anterior and posterior bins, defined by a division at Bregma level -2.60 mm (Tanti et al., 2012; Tanti and Belzung, 2013; Zhou et al., 2016). Bregma level -2.60 mm was identified based on when hippocampal CA3 reaches approximately halfway down the dorsal/ventral extent of the brain, and the corpus callosum no longer connects the left and right hemispheres (Paxinos and Franklin, 2019). Cell counts for each section anterior to -2.60 mm were summed and multiplied by 9 to get an anterior value for each mouse. The same procedure was used for the posterior analysis, using all sections posterior to Bregma level -2.60 mm.