Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Creation of Tet-inducible, p53-expressing, CRISPR/Cas9-edited stable cell lines
Request a Protocol
Ask a
question
Favorite
After identification and expansion of the desired 4-bp mutation clones, Tet-inducible p53-expressing, “BAX-4bp” stable H1299 cell lines were generated as previously described, via lentiviral infection using a ViraPower HiPerform Lentiviral Expression System (Invitrogen)18. WT and K120R-p53 protein expression was induced by treatment of TetR-expressing “BAX_4bp” cells with 1 μg/ml of Tetracycline (Millipore Sigma).
Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
