Single Guide RNA (sgRNA) Design

CK Christopher Kressler
GG Gilles Gasparoni
KN Karl Nordström
DH Dania Hamo
AS Abdulrahman Salhab
CD Christoforos Dimitropoulos
ST Sascha Tierling
PR Petra Reinke
HV Hans-Dieter Volk
JW Jörn Walter
AH Alf Hamann
JP Julia K. Polansky
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Twenty nt guide sequences were designed using Benchling (www.benchling.com) and sequences covering the majority of CpGs in the TSDR with high Off-Target scores (min >50, mostly >70) were chosen.

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