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Lactate dehydrogenase

JW Jian-jun Wen
XW Xianxiu Wan
JT John Thacker
NG Nisha Jain Garg
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Lactate dehydrogenase (LDH) activity in tissue homogenates and plasma was measured by using a 2-step kit (Cayman Chemicals, Ann Arbor, Michigan). Briefly, LDH-catalyzed reduction of NAD+ to NADH by oxidation of lactate to pyruvate was coupled with reduction of tetrazolium salt to blue formazan crystals, and absorbance was monitored at 490 to 520 nm by spectrophotometry (standard curve 1 to 1,000 μU of LDH).

Copyright and License information:  ©2016 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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