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DNA sequences of Wnt and E-cadherin were amplified using human bone cells cDNA library, and cloned into a plasmid expression vector pcDNA3.1 (Invitrogen, Carlsbad, CA, USA) to generate Wnt overexpressing (pWnt) or E-cadherin overexpressing plasmids (pE-cad). The miR-128 knockdown Mg63 cells were transfected with pWnt, pE-cad, or a control empty plasmid (Control) using a Lipofectamine 3000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Cells were used for further analyses after 72 h of transfection.
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