Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

LncRNA target gene prediction

HL Hao Li
PC Pengfei Cui
XF Xue Fu
LZ Lan Zhang
WY Wenjun Yan
YZ Yaru Zhai
CL Changwei Lei
HW Hongning Wang
XY Xin Yang
request Request a Protocol
ask Ask a question
Favorite

The target gene prediction of lncRNAs was divided into cis-target gene prediction and trans-target gene prediction. We screened for genes with protein-coding capabilities within 100 kb upstream and downstream of lncRNAs for cis-regulated target genes corresponding to lncRNAs. The target genes with trans-regulation were screened by calculating the expression correlation (Pearson’s correlation test) or co-expression analysis method between lncRNAs and mRNAs. We used the correlation coefficient > 0.95 and p-value < 0.05 to screen the trans-acting relationship between lncRNAs and mRNAs. Next, the lncRNA-mRNA co-expression network was constructed using Cytoscape.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A