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Determination of Encapsulation Efficiency and Drug Loading

WB Wei-Lian Bao
QW Qibiao Wu
BH Bin Hu
DS Dongdong Sun
SZ Shengnan Zhao
XS Xiaoyan Shen
HC Haibo Cheng
WS Weixing Shen
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The supernatant was collected when the nanoparticles were prepared, the volume of the solution was accurately determined, and the absorbance of the solution was measured by an ultraviolet spectrophotometer to obtain the content of the SRP-NPs free from the supernatant. Encapsulation efficiency was calculated according to the formula:

EN, entrapment efficiency; Cf, Free drug concentration; Ct, Total concentration.

The drug loading was calculated according to the formula:

LE, percentage of drug loaded in the liposomes; We, dose encapsulated in the liposomes; Wm, the total weight of the drug loaded liposomes.

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1 Q&A

Encapsulation efficiency- weight of the free drug?
edit 1 Answer 79 Views Jun 19, 2022