The supernatant was collected when the nanoparticles were prepared, the volume of the solution was accurately determined, and the absorbance of the solution was measured by an ultraviolet spectrophotometer to obtain the content of the SRP-NPs free from the supernatant. Encapsulation efficiency was calculated according to the formula:
EN, entrapment efficiency; Cf, Free drug concentration; Ct, Total concentration.
The drug loading was calculated according to the formula:
LE, percentage of drug loaded in the liposomes; We, dose encapsulated in the liposomes; Wm, the total weight of the drug loaded liposomes.
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