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On days 0, 14 and 28, 0.2 ml of blood samples were collected from the eye socket of each mouse 2 h after oral gavage with a probiotic or water. After incubation for 1 h at 25˚C, serum was obtained and centrifuged at 2,000 x g 4˚C for 10 min. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels were determined using an automatic analyzer (Hitachi 7080; Hitachi, Ltd.) at the National Laboratory Animal Center (Taipei, Taiwan).
Copyright and License information: ©2021 Hsieh et al.
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