Xenograft Mouse Model

A total of 20 male BALB/c mice were assigned to 4 groups (5 mice/group) and maintained under pathogen-free conditions at room temperature (21 to 25°C), with 12 h–12 h light–dark cycle. Food and water were offered ad libitum. A total of 5 × 10⁶ human NCI-H1975 cells suspended in PBS were subcutaneously injected into the right front leg of 6-week-old male BALB/c mice. When the tumors reached 100–150 mm³, 100 or 200 mg/kg/day (calculated according to clinical condition and converted using formula, ie, mouse dosage = X mg/kg×70 kg×0.0026/20 g = 9.1 ×X mg/kg) of the extract of the physalins was mixed with 0.5% CMC-Na as a suspension and administered orally once a day for 12 days in the physalin-treated groups, and 0.5% CMC-Na was administered orally to the control group. In the cisplatin (DDP) treated group, 5 mg/kg/day DDP was intraperitoneally injected for 12 days. The tumor volume and body weight were assessed every two days. The tumor volume was calculated as V = length × width²/2.¹⁵ The mice were sacrificed quickly by cervical dislocation, and then, the tumors were obtained and measured.