The sample was prepared following the protocol used by Murtey and Ramasamy (2016) and was centrifuged to obtain solid pallet of bacterial cells of three clinical strains [51]. The pallet was washed twice and dehydrated with a gradient solvent of ethanol for 20 minutes. This examination was done to check the potency of compound 11 with cefixime at 1:1 μg/μl and control with blank sample.
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