The amount of FBG, total cholesterol (TC), triglycerides (TG), LDL- cholesterol (LDL-C) and HDL cholesterol [HDL-C] in serum were estimated, using commercial reagent kits by fully automated clinical chemistry analyzer (ChemWell Awareness Technology, USA). The indices of atherogenic lipid risk factors were assessed, by calculating TC/HDL, TG/HDL, LDL/HDL and Atherogenic index of plasma (AIP) [log10 (TG/HDL-c)], as described previously [21, 22]. NSE was estimated by using ELISA method (Fine Test kit, Wuhan Fine Biotech Co., Ltd, Wuhan, China).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.