2.2. Drugs

PF-3845 (selective FAAH inhibitor), JZL184 (selective MAGL inhibitor) and JZL195 (dual FAAH/MAGL inhibitor) were synthesized and provided by the Cravatt Laboratory. Each compound was dissolved in a vehicle of ethanol:emulphor:saline (1:1:18) and injected intraperitoneally (i.p.) in a volume of 10 mL/kg body weight. The following doses were used for behavioral tests (PF-3845: 1, 3 and 10 mg/kg; JZL184: 2, 10 and 40 mg/kg; and JZL195: 3, 8, 20 mg/kg) and in vivo microdialysis (PF-3845: 3 mg/kg; and JZL184: 10 mg/kg). Pretreatment times were 180min for PF-3845 and JZL195, and 60min for JZL184 based on the temporal profiles for efficacious enzyme inhibition (Ahn et al., 2009; Long et al., 2009a, 2009c). The number of animals per group size is indicated for each experiment in the figure legends.