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Fatty acid uptake assay

JK Jihyun Kim
JM Jiyoung Moon
CP Chul-Hong Park
JL Jisu Lee
HC Helia Cheng
ZF Z. Elizabeth Floyd
JC Ji Suk Chang
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Uptake of [14C]-palmitic acids by tissue explants and cells was measured as described previously42 with slight modification. In short, tissue pieces or cells were incubated in HBS solution containing 0.1% fatty acid-free BSA and [14C]-palmitic acids (2 µM) at 37 °C for 15 min or on ice followed by washing four times with ice-cold HBS solution containing 0.1% fatty acid-free BSA. The tissue pieces and cells were then lysed in RIPA buffer and the supernatant was used for liquid scintillation counting. The small amount of extracellular [14C]-palmitate remaining was corrected from the [14C]-palmitate content in samples. Cellular [14C]-palmitate uptake was normalized to total protein content in each sample.

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