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2.6. ALDH2 activity assay after Alda-1 treatment
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To activate ALDH2, cardiomyocytes were treated with Alda-1 (20 μM, Sigma-Aldrich, St. Louis, MO, USA) for 12 h [19,30,31]. ALDH2 activity was detected by the assay kit from GENMED SCIENTIFICS. Normal and ALDH2-activated mitochondria were collected for further analyses.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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