2.2. Preparation of Ananas comosus fruit peel extract

Ananas comosus (pineapple) was bought from Oje Market, Ibadan, the Oyo State capital, Nigeria. A. comosus was peeled in the lab using a sterile knife, the peel was chopped into smaller pieces, and then oven dried at 40 °C for 72 h. Dried peel were ground using a mechanical grinder. 200 g of the powdered peel was soaked in 80% methanol for 48 h. The extract was filtered using a muslin cloth and doubly filtered with whatmann filter paper. The filtrate was concentrated under reduced pressure in rotary evaporator and dried further in vacuum over to a constant weight. The extract was greenish brown semi solid, tagged Peel Extract of A. comosus (PEAC).