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Virus vectors

FF Felipe Fredes
MS Maria Alejandra Silva
PK Peter Koppensteiner
KK Kenta Kobayashi
MJ Maximilian Joesch
RS Ryuichi Shigemoto
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AAV5-hSyn-DIO-mCherry, AAV5-hSynDIO-hM4Di-mCherry and AAV5-hSyn-DIO-hM3Dq-mCherry vectors were obtained from University of North Carolina vector core and Addgene viral service. AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 and AAV5-hSyn-Flex-GCaMP6s-WPRE-SV40 were obtained from University of Pennsylvania Vector Core. pAAV1-CAG-Vamp2-venus transfer plasmid was generated by subcloning VAMP2-venus gene cassette obtained from Hiromu Yawo into pAAV-CaMKII-GFP (Addgene). Plasmid AAV-CAG::FLEX-rev::ChR2-HA-2a-hM4D was obtained from Addgene. Viral titrations were between 8 × 1012 and 7.5 × 1013 genome copy per ml. For inducing expression in Prox1-creERT2 animals, tamoxifen (T5648, Sigma) previously diluted in corn oil was injected i.p. (75 mg/kg body weight) for 5 consecutive days.

Copyright and License information:  ©2020 The Author(s)
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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