2.5. In vitro release of BBR and 10-HCPT

The in vitro release behavior of BBR-10-HCPT-loaded LM was evaluated by using the dialysis method. 0.5 mL BBR-10-HCPT-loaded LM was added to the dialysis bag and the molecular weight cutoff was 3500 g/mol. 50.0 mL PBS solution (pH 7.4, 37 °C) containing 0.5% tween 80 and 40% rat plasma were (obtained by dilution with physiological saline) used as dialysis fluid and the vibration speed was 100.0 rpm. 0.5 mL aliquot was withdrawn at 0.5 h, 1.0 h, 2.0 h, 4.0 h, 6.0 h, 8.0 h, 24.0 h, 48.0 h, and 72.0 h separately, and then 0.5 mL fresh medium was added. The concentration of BBR and 10-HCPT was determined by HPLC-MS/MS.