Genome sequencing

The genomes of the three PlxyGV isolates were sequenced by using Illumina Hisen X ten system. Sequence assembly were done by using SOAP denovo (Version 2.04) software (BGI), and using the first published genome sequence of the PlxyGV-K1 isolated from Japan ({"type":"entrez-nucleotide","attrs":{"text":"NC_002593","term_id":"11068003","term_text":"NC_002593"}}NC_002593) as a reference. PCR was performed to synthesize DNA fragments bridging the gaps between contigs by using the genomic DNAs of individual PlxyGV isolates as templates. PCR products were sequenced from both ends. The sequences were assembled with the initial contigs into a single, circular contig. Sequences were analyzed with Lasergene programs (DNASTAR). Homology searches were carried out with GenBank/EMBL, SWISSPROT and PIR databases by using the BLAST algorithm. Multiple sequence alignments were performed by using CLUSTAL W. The PlxyGV genome sequence accession numbers are {"type":"entrez-nucleotide","attrs":{"text":"MN099284","term_id":"1859766653","term_text":"MN099284"}}MN099284 for PlxyGV-W, {"type":"entrez-nucleotide","attrs":{"text":"MN099285","term_id":"1859766772","term_text":"MN099285"}}MN099285 for PlxyGV-B and {"type":"entrez-nucleotide","attrs":{"text":"MN099286","term_id":"1859766891","term_text":"MN099286"}}MN099286 for PlxyGV-Wn.