106 Y6 iPSC cells were collected by collagenase treatment and resuspended in 100 ml of DMEM/F12, collagen, and Matrigel mix (2:1:1 ratio). Cells were injected i.m. into immunodeficient Rag2−/−GammaC−/− mice. After 8 wk, teratomas were harvested, fixed, and subjected to paraffin embedding and H&E staining.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.