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A total of 2.5 × 106 WT and NFATc3-KO SMMC7721 cells were injected into underarm skin on each side of the same nude mouse simultaneously (6-week old, female), then the mice were monitored for tumor forming every 3 days, tumor dimensions were measured twice a week and tumor volume was calculated using Volume = π/6×(length)×(width)2. After eight weeks, the mice were sacrificed and tumors were obtained for measuring the sizes and weights of tumors. All experiments in mice were conducted according to institutional guidelines of Peking University Health Science Center.
Copyright and License information: ©2021 The Author(s). Published with license by Taylor & Francis Group, LLC.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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