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DAPI staining

MC Minghao Chia
CL Cai Li
SM Sueli Marques
VP Vicente Pelechano
NL Nicholas M. Luscombe
FW Folkert J. van Werven
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The rate of meiotic divisions was monitored by DAPI staining as previously described [41]. Cells were pelleted by centrifugation (~ 2400g, 1 min, room temperature) and fixed in 80% (vol/vol) ethanol for at least 60 min before further processing. Subsequently, samples were pelleted by centrifugation (~ 2400g, 1 min) and re-suspended in PBS with DAPI (1 μg/ml). Cells were sonicated for a few seconds and left in the dark at room temperature for at least 5 min. After DAPI staining, the proportion of cells containing one, two, three, or four DAPI masses were counted using a fluorescence microscope.

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