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Tumor xenografts in nude mice

YZ Yahui Zheng
CH Chong Huang
LL Lu Lu
KY Kangkang Yu
JZ Jing Zhao
MC Mingquan Chen
LL Lu Liu
QS Qingfeng Sun
ZL Zhifei Lin
JZ Jianming Zheng
JC Jinhong Chen
JZ Jubo Zhang
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All experimental procedures involving animals were approved by The Animal Care and Use Committee of Fudan University, China. Five-week-old nude mice (BALB/c) were randomly divided into indicated groups (n = 5 per group) before inoculation or injection. HCCLM3-shNC and HCCLM3-shSTOML2 cells were subcutaneously injected into the mice (1.0 × 107 cells/mouse) to form the subcutaneous model. For the xenograft model, subcutaneous tumors were removed and dissected into 1 mm3 sections, which were incubated into the liver parenchyma of nude mice. For drug treated groups, mice were injected intraperitoneally with hydroxychloroquine (HCQ, 50 mg/kg), lenvatinib (LV, 5 mg/kg or 10 mg/kg), combination with lenvatinib (5 mg/kg) and HCQ (50 mg/kg) or saline as control. The mice were sacrificed at a time-defined endpoint and tumor volume, weight, and number of metastatic nodules were assessed by double-blinded evaluation. The details are presented in Additional file 1: supplementary materials and methods.

Copyright and License information: The Author(s) ©2021
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