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Preparation of a UUO model and MSC administration
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A UUO model, which is an experimental renal fibrosis model, was prepared to investigate the anti-fibrotic effect of MSCs. Rats were anesthetized by the same procedure described for the IRI model. The left ureter was exposed after an abdominal midline incision and ligated to induce UUO. At 4 days after the UUO operation, PBS, control rMSCs, or IFN-γ rMSCs (2.5 × 106 cells/rat) were injected into the tail vein. After 7 days, the rats were sacrificed and examined for renal fibrosis.
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