RNA extraction, quantification, and qualitative analysis

CI Chisaki Ikeda
HH Hiroaki Haga
NM Naohiko Makino
TI Tatsutoshi Inuzuka
AK Ayako Kurimoto
TU Toshiki Ueda
AM Akiko Matsuda
YK Yasuharu Kakizaki
TI Tetsuya Ishizawa
TK Toshikazu Kobayashi
SS Shinpei Sugahara
MT Michihiko Tsunoda
KS Kensei Suda
YU Yoshiyuki Ueno
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MicroRNA was extracted from 200 µL EVs using the miRNeasy serum/plasma kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. The RNA concentration was measured using a microRNA assay kit with a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA., USA). The quality of the small RNA was assessed using an Agilent 2100 Bioanalyzer and an Agilent Small RNA kit (Agilent Technologies, Santa Clara, CA, USA). Electropherograms were visualized using Agilent 2100 Expert software (Agilent Technologies).

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