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Lysotracker RED and lysosensor GREEN staining
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The cells stained with 0.5 μM lysotracker RED for 45 min in cell culture medium at 37 °C were analyzed for the lysosomal mass. For analyzing the lysosomal pH, the cells were stained with 1 μM lysosensor GREEN for 20 min in cell culture medium at 37 °C. After washing with PBS, the processed cells were suspended and analyzed by flow cytometry or detected by fluorescence microscope.
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