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Keratinocyte colony counting (proliferation) assay
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Keratinocytes in passage 2 were seeded (24,000 cells/cm2) in either complete growth medium containing 0.1% DMSO (control group); or complete growth medium with 0.025% Omega-7 dissolved in 0.1% DMSO (treatment group) onto a fresh feeder layer of mitomycin C-treated 3T3 fibroblasts48 that had been prepared 24 h earlier at the same cell density as the keratinocytes in 6 well plates. The cells were cultured for 96 h at the same conditions described above. The number of colonies in 5 fields per well were counted at 72 and 96 h after treatment.
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