Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Crude fibre determination

SS Serifat Olatundun Salami
AA Anthony Jide Afolayan
request Request a Protocol
ask Ask a question
Favorite

This was determined as described by49 with little modification. A glass crucible containing 2 g of the sample and attached to the extraction unit, boiling 1.25% sulphuric acid and solution (150 ml) was then added. The sample was digested for 30 min, after which the acid was drained out, and boiling distilled water was used to rinse the residue four times. The residue was then rinsed using 100 ml of 1.25% NaOH solution. Finally, the crucible was removed from the extraction unit and oven-dried at 110 °C (Overnight), allowed to cool in a desiccator and weighed (W1). Ashing of the sample was then done in a muffle furnace at 550 °C for five hours cooled in a desiccator and reweighed (W2).

The percentage of crude fibre was calculated using the following formula:

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A