From the GISAID repository31, we downloaded 53,183 sequences available on August 10th, for SARS-CoV-2, from different countries, from there 52,645 have as Ns, high coverage and host = “homo sapiens”. Then, we calculated the frequency table of the 21-bps sequences obtained from experiments 2 and 3, to verify which sequences remain and could be used for detection. The appearance frequency of the target sequences among the samples in the GISAID dataset is reported in Table 1, second column. In addition, we downloaded 26 sequences from GISAID repository of other hosts (manis javanica, rhinolophus affinis, canine and felis catus) to make a comparison in the sequences from experiment 2 and 3.
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