In order to analyze developmental differences Cx. quinquefasciatus lines were compared. Following Baton et al.31 3 variables were considered to study mosquito fitness (w+ and w−): survival, fecundity and fertility. For that, egg laid, hatching rate, developmental stages and individual survivor number per line were daily monitored. Four rafts of eggs from each line were placed in individual containers with dechlorinated water until neonates hatched. Larvae were provided with commercial fish food (Shulet Carassius) to pupal stage. Eggs, larvae, pupae, and adults were quantified under a binocular microscope (Nikon SMZ800) until adults emerged. The full experiment was repeated 4 times considering four different generations of mosquito lines from the laboratory.
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