Growth curve

Leonardo Lonati; Sofia Barbieri; Isabella Guardamagna; Andrea Ottolenghi; Giorgio Baiocco

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

Growth curve

LL Leonardo Lonati

SB Sofia Barbieri

IG Isabella Guardamagna

AO Andrea Ottolenghi

GB Giorgio Baiocco

This method is extracted from research article: Sci Rep, Jan 2021

Radiation-induced cell cycle perturbations: a computational tool validated with flow-cytometry data

DOI: 10.1038/s41598-020-79934-3

Request a Protocol

Ask a question

Favorite

Independent measurements on cellular growth curves were performed in order to estimate the doubling time ( $T_{D}$ ) of an unirradiated population. $10^{5}$ cells were seeded per T25 flask, they were cultivated and harvested at the following time points: 6 h, 16 h, 24 h, 48 h, 72 h (the same time points for the following study of the radiation effects). The samples were trypsinized, $10 μ l$ of the pellet was placed in a Bürker counting chamber. The number of cells were counted under an inverted microscope. Experiments were repeated in biological triplicate, each of them was performed in technical duplicate. The data were fitted to the following exponential function via non-linear least squares methods: $f i t (t) = N_{0} exp (γ t)$ , where $N_{0}$ is the number of cells at $t = 0$ , and $γ$ is the growth rate.

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol