EAE induction

EAE was induced by subcutaneous immunization of SJL/J mice with proteolipid protein peptide (PLP_139-151) and C57BL/6 mice with myelin oligodendrocyte glycoprotein peptide (MOG_35-55); for both peptides 250 µg peptide (Pepceuticals, Leicester, UK) per animal were emulsified with M. Tuberculosis H37RA (List Biological Laboratories, Campbell, US, 800 µg/animal) in 100 µL Complete Freund's Adjuvant (BD Difco, Heidelberg, Germany). Pertussis Toxin (Biotrend, Cologne, Germany, 1.25 ng/µL in SJL/J and 2 ng/µL in C57BL/6) was administered intraperitoneally in 200 µL PBS on days 0 and 2 ⁵⁸.

EAE scoring was performed daily: righting reflex weakness = 0.5, tail paresis = 0.5, tail paralysis = 1, unilateral hindlimb paresis = 0.5, bilateral hindlimb paralysis = 1, unilateral forelimb paresis = 0.75, bilateral forelimb paralysis = 1.5.