Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Cells were treated with vehicle (DMSO) or Evo for three days and then harvested by trypsinization. Live cells, as confirmed using a trypan blue exclusion assay, were diluted in PBS, mixed with Matrigel (ratio 1:1), and then inoculated into the right flanks of NOD/SCID mice. The incidence of tumor formation was determined. Tumor-initiation fraction of vehicle- or Evo-treated groups was determined by using Extreme Limiting Dilution Analysis (ELDA) online software (http://bioinf.wehi.edu.au/software/elda/) 35.
Copyright and License information: ©2021 The author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
